HiSeq 3000/4000 SBS Kit

Includes sequencing reagents specifically designed for the HiSeq 3000/HiSeq 4000 Systems. Enables production-scale sequencing for diverse applications.

Support and reagent availability for HiSeq 3000 Systems ended February 28, 2023. Support and reagent availability for HiSeq 4000, certified used (CPO) HiSeq 4000 and cBot 2 Cluster generation Systems ended March 31, 2024. to see if you qualify for a trade–in.

Overview

HiSeq 3000/4000 SBS (Sequencing by Synthesis) Kits support a wide range of sequencing applications, making them ideal for high-throughput labs performing various genomic applications. Compatible with the HiSeq 3000 and HiSeq 4000 Sequencing Systems, the new kits leverage patterned flow cell technology for even cluster spacing and uniform feature size, to deliver extremely high cluster densities and significant increases in data output and daily throughput.

The HiSeq 3000/4000 SBS Kits enable up to 1500 Gb (1.5 Tb) of output per dual flow cell run when used with the HiSeq 3000/4000 PE Cluster Kit. This high capacity means that more samples can be sequenced simultaneously at a great depth, generating rich and meaningful data.

This product is available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.


Specifications

Applications

Related applications and methods

Documentation

Product literature

Literature is not currently available for this product.

Figures

Advanced patterned flow cell design enables maximum throughput

Patterned flow cells contain billions of nanowells at fixed locations, providing even cluster spacing and uniform feature size, and delivering extremely high cluster densities.

Resources

Sequencing reagent performance depends on thawing technique, thawed storage time, and storage conditions. Learn how to thaw and store sequencing reagents for optimal performance.

HiSeq 3000/4000 SBS Kit (50 cycles)

FC-410-1001

Includes sequencing reagents for one flow cell to support a 50-cycle run on the HiSeq 3000/4000 Systems. Purchase the HiSeq 3000/4000 Cluster Kits separately.

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HiSeq 3000/4000 SBS Kit (150 cycles)

FC-410-1002

Includes sequencing reagents for one flow cell to support a 150-cycle run on the HiSeq 3000/4000 Systems. Purchase the HiSeq 3000/4000 Cluster Kits separately.

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HiSeq 3000/4000 SBS Kit (300 cycles)

FC-410-1003

Includes sequencing reagents for one flow cell to support a 300-cycle run on the HiSeq 3000/4000 Systems. Purchase the HiSeq 3000/4000 Cluster Kits separately.

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Illumina Advantage Products (5)

TG HiSeq® 3000/4000 SBS Kit (50 cycles)

TG-410-1001

Provides kitted reagents for up to 50 cycles of sequencing and sufficient reagents to support dual-indexing protocols on the HiSeq 3000/4000 sequencing systems.

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TG HiSeq® 3000/4000 SBS Kit (150 cycles)

TG-410-1002

Provides kitted reagents for up to 150 cycles of sequencing and sufficient reagents to support dual-indexing protocols on the HiSeq 3000/4000 sequencing systems.

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TG HiSeq® 3000/4000 SBS Kit (300 cycles)

TG-410-1003

Provides kitted reagents for up to 300 cycles of sequencing and sufficient reagents to support dual-indexing protocols on the HiSeq 3000/4000 sequencing systems.

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TG HiSeq® 3000/4000 PE ClusterKit

TG-410-1004

TG HiSeq® 3000/4000 PE ClusterKit.

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TG HiSeq® 3000/4000 SR ClusterKit

TG-410-1005

TG HiSeq® 3000/4000 SR ClusterKit

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Trade in your HiSeq instrument for more discovery power

If you currently own a HiSeq 2500, HiSeq 3000/4000, or HiSeq X, you may be eligible to trade in your existing instrument for a new NovaSeq 6000 System. This program is available for a limited time only. Terms and conditions apply. Fill out the form below to contact a sales representative to see if you are eligible.

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