Pillar® oncoReveal™ BRCA1 & BRCA2 + CNV Panel

A targeted BRCA1/2 gene panel, utilizing the single-tube SLIMamp™ workflow for fast library prep and turnaround time.

Overview

Pillar® oncoReveal™ BRCA1 & BRCA2 + CNV Panel is targeted for BRCA1/2 mutations and copy number variations. The assay uses proprietary Stem Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.

  • Maintain control of samples and results with single-tube, tiled amplification that can be performed in-house by any NGS lab

  • Achieve variant detection as low as 1% VAF without UIDs, even with limited DNA input or poor sample quality

  • Improve lab efficiency and reduce “no calls”, repeat testing and difficult interpretation decisions

  • < 8 hours DNA to sequencer

Specifications

Required Products

Pillar® oncoReveal™ panels require the use of a Pillar index kit.

Pillar® oncoReveal™ panels require the use of a Pillar index kit. Pick your preferred number of reactions.

/ Results

Applications

Example workflow

1
Prepare Library for:

Pillar® oncoReveal™ BRCA1 & BRCA2 + CNV Panel

2
Sequence Efficiently with:

Documentation

Product literature

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Support documentation

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Pillar® oncoReveal™ BRCA1 & BRCA2 + CNV Panel AmpliSeq for Illumina BRCA Panel
Content specifications Covers full coding gene sequence, plus flanking intronic regions (10bp) Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2
Instruments MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System
Method Targeted DNA sequencing, Amplicon sequencing Targeted DNA sequencing, Amplicon sequencing
Nucleic acid type DNA DNA
Specialized sample types Blood, Low-input samples, FFPE tissue Blood, FFPE tissue
Species category Human Human
Technology Sequencing Sequencing
Variant class Somatic variants, Germline variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs) Single nucleotide polymorphisms (SNPs), Somatic variants, Germline variants, Insertions-deletions (indels)

Figures

Panel specifications

Pillar® oncoReveal™ BRCA1 & BRCA2 + CNV Panel (24 reactions)

HDA-BR-1003-24

Pillar® oncoReveal™ BRCA1 & BRCA2 + CNV Panel (24 reactions)

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Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

IDX-PI-1001-96

Indices PI501-8, PI701-4 (32 combinations, 96 reactions)

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Kit D, indices PI501-8, PI701-12

IDX-PI-1004-192

Kit D, indices PI501-8, PI701-12

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