Assay time |
~3-4 hr (from DNA extraction to normalized library) |
5 hr total assay time |
~6 hr total assay time
|
~5.5 hr from DNA extraction to normalized library. (Library prep time: ~90 minutes). |
Automation capability |
Liquid Handling Robots |
Liquid Handling Robots |
Liquid Handling Robots |
Liquid Handling Robots |
Description |
A fast, flexible library prep workflow that accommodates an assortment of sample types and DNA input amounts, allowing access to a wide range of applications, from human to microbial whole-genome sequencing and more.
|
A simple, all-inclusive PCR-free prep for whole-genome sequencing studies with the ability to sequence through challenging regions of the genome. |
A low-input research method that delivers high genome coverage quality and reduced bias. |
Prepare sequencing libraries for small genomes, PCR amplicons, and plasmids in 90 min, with a low DNA input requirement. |
Hands-on time |
1-1.5 hr |
4 hr |
~4 hr |
15 min |
Input quantity |
1-500 ng DNA for small genomes (e.g. microbial), or 100-500 ng DNA for large genomes (e.g. human). (For blood and saliva, see the reference guide). |
1 ug DNA |
100 ng genomic DNA |
1 ng DNA |
Mechanism of action |
Bead-linked transposome |
Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. |
Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free. |
Enzymatic fragmentation |
Method |
Shotgun Sequencing,
Whole-Genome Sequencing,
De Novo Sequencing,
Amplicon Sequencing |
Shotgun Sequencing,
Whole-Genome Sequencing,
Genotyping by Sequencing |
Shotgun Sequencing,
Whole-Genome Sequencing,
Genotyping by Sequencing |
Shotgun Sequencing,
Whole-Genome Sequencing,
De Novo Sequencing,
16s rRNA Sequencing,
Amplicon Sequencing |
Multiplexing |
Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations |
Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations |
Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations |
Up to 384 uniquely indexed samples may be pooled and sequenced together. |
Nucleic acid type |
DNA |
DNA |
DNA |
DNA |
Specialized sample types |
Blood,
Not FFPE-Compatible,
Saliva |
Not FFPE-Compatible |
Not FFPE-Compatible,
Low-Input Samples |
Not FFPE-Compatible,
Low-Input Samples,
Single Cells |
Species category |
Drosophila,
Mammalian,
Mouse,
Yeast,
Zebrafish,
Human,
Rat,
Plant,
Virus,
Nematode,
Bacteria,
Any Species |
Other,
Mammalian,
Mouse,
Human,
Rat,
Plant |
Other,
Mammalian,
Mouse,
Human,
Rat,
Plant |
Drosophila,
Mammalian,
Mouse,
Yeast,
Zebrafish,
Fungal,
Human,
Rat,
Plant,
Virus,
Nematode,
Bacteria,
Any Species |
Species details |
Compatible with any species |
Compatible with most large DNA genomes. |
Compatible with most large DNA genomes. |
Compatible with any species |
Systems |
MiSeq System,
iSeq 100 System,
NextSeq 2000 System,
NextSeq 1000 System,
MiSeqDx in Research Mode,
MiniSeq System,
NextSeq 550Dx in Research Mode,
NovaSeq X System,
NovaSeq 6000 System,
NovaSeq X Plus System,
NextSeq 550 System |
MiSeq System,
NextSeq 2000 System,
NextSeq 1000 System,
MiSeqDx in Research Mode,
MiniSeq System,
NovaSeq X System,
NextSeq 500 System,
NovaSeq 6000 System,
NovaSeq X Plus System,
NextSeq 550 System |
MiSeq System,
NextSeq 2000 System,
MiSeqDx in Research Mode,
MiniSeq System,
NovaSeq X System,
NextSeq 500 System,
NovaSeq 6000 System,
NovaSeq X Plus System,
NextSeq 550 System |
MiSeq System,
iSeq 100 System,
NextSeq 2000 System,
NextSeq 1000 System,
MiniSeq System,
NextSeq 500 System,
NextSeq 550 System |
Target insert size |
~350 bp |
350 bp or 550 bp |
350 bp or 550 bp |
300 bp–1.5 kb |
Technology |
Sequencing |
Sequencing |
Sequencing |
Sequencing |