A robust assay targeting key mutations in MPL, JAK2 and CALR genes, utilizing single-tube SLIMamp workflow for fast library prep and turnaround time.
Assay time
Hands-on time
Input quantity
The Pillar® oncoReveal™ Essential MPN Panel is a robust assay that simultaneously tests for key mutations within the MPL, JAK2 and CALR genes. The assay uses proprietary Stem Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.
| Assay time | ~6.5 hr |
|---|---|
| Cancer type | Hematologic |
| Description | A targeted gene panel focused on mutations affecting MPL, JAK2 and CALR, simple single tube workflow |
| Hands-on time | ~3 hr |
| Input quantity | 5-80ng DNA, 10-50ng RNA |
| Instruments | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode |
| Mechanism of action | Multiplex PCR using tiled amplicon |
| Method | Targeted DNA sequencing, Amplicon sequencing |
| Nucleic acid type | DNA |
| Number of amplicons | 3 genes, 7 amplicons |
| Number of reactions | 48 reactions per kit |
| On-target reads | 99.9% ± 0.0% |
| Specialized sample types | Blood, Low-input samples |
| Species category | Human |
| Target insert size | Average: 167bp |
| Technology | Sequencing |
| Uniformity | 100% |
| Variant class | Somatic variants, Single nucleotide variants (SNVs), Insertions-deletions (indels) |
Pillar® oncoReveal™ panels require the use of a Pillar index kit. Pick your preferred number of reactions.
Pillar® oncoReveal™ Essential MPN Panel
Literature is not currently available for this product.
| Pillar® oncoReveal™ Essential MPN Panel | AmpliSeq for Illumina Myeloid Panel | TruSight Myeloid Sequencing Panel | |
|---|---|---|---|
| Assay time | ~6.5 hr | 5.5 – 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 8 hours |
| Cancer type | Hematologic | Hematologic | Hematologic |
| Description | A targeted gene panel focused on mutations affecting MPL, JAK2 and CALR, simple single tube workflow | Targeted panel to study 40 DNA genes, 29 RNA fusion driver genes, and 5 gene expression levels associated with myeloid cancers. | For somatic mutation detection studies in myeloid malignancies. |
| Hands-on time | ~3 hr | < 1.5 hr | 3 hr |
| Input quantity | 5-80ng DNA, 10-50ng RNA |
20 ng high-quality DNA; 10 ng high-quality RNA (10 ng recommended per pool) |
50 ng |
| Instruments | MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, NextSeq 550Dx in Research Mode | MiSeq System, MiniSeq System | MiSeq System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System |
| Mechanism of action | Multiplex PCR using tiled amplicon | ||
| Method | Targeted DNA sequencing, Amplicon sequencing | Targeted DNA sequencing, Amplicon sequencing, Targeted RNA sequencing | Targeted DNA sequencing, Amplicon sequencing |
| Nucleic acid type | DNA | DNA, RNA | DNA |
| Number of amplicons | 3 genes, 7 amplicons | ||
| Number of reactions | 48 reactions per kit | ||
| On-target reads | 99.9% ± 0.0% | ||
| Specialized sample types | Blood, Low-input samples | Blood, Not FFPE-compatible, Bone marrow | Not FFPE-compatible, Not FFPE-compatible |
| Species category | Human | Human | Human |
| Target insert size | Average: 167bp | ||
| Technology | Sequencing | Sequencing | Sequencing |
| Uniformity | 100% | ||
| Variant class | Somatic variants, Single nucleotide variants (SNVs), Insertions-deletions (indels) | Single nucleotide polymorphisms (SNPs), Gene fusions, Somatic variants, Insertions-deletions (indels) | Somatic variants, Insertions-deletions (indels) |
Gene list
Panel specifications
Pillar® oncoReveal™ Essential MPN Panel (48 reactions)
HDA-MY-1002-48
Pillar® oncoReveal™ Essential MPN Panel (48 reactions)
Indices PI501-8, PI701-4 (32 combinations, 96 reactions)
IDX-PI-1001-96
Indices PI501-8, PI701-4 (32 combinations, 96 reactions)
Kit D, indices PI501-8, PI701-12
IDX-PI-1004-192
Kit D, indices PI501-8, PI701-12
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