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Nextera XT DNA Library Preparation Kit

Description 1

Nextera XT DNA

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Nextera XT DNA Library Preparation Kit

Description 2

Nextera XT DNA Library Preparation Kit

Nextera XT DNA Library Preparation Kit

These expert-defined sequencing research panels target 94 genes and 284 SNPs associated with a predisposition towards various cancers.

Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement.

~5.5 hr from DNA extraction to normalized library. (Library prep time: ~90 minutes).

Assay time

15 min

Hands-on time

1 ng genomic DNA (compatible with most large DNA genomes)

Input quantity

See full details in the specifications table

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Support and reagent availability for HiSeq 3000 Systems ends February 28, 2023. Support and reagent availability for HiSeq 4000, certified used (CPO) HiSeq 4000 and cBot 2 Cluster generation Systems ends March 31, 2024. Contact us to see if you qualify for a trade–in.

Overview

The Nextera XT DNA Library Preparation Kit offers a fast and easy workflow for preparing, quantifying, and normalizing libraries for sequencing small genomes, PCR amplicons, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA from a low amount of input DNA (1 ng), making this kit amenable for use with precious samples available in limited quantity.
This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

Key features of the Nextera XT DNA Library Preparation Kit

  • Provides rapid library preparation in as little as 90 min with only 15 min of hands-on time

  • Eliminates library quantification before sample pooling and sequencing

  • Delivers quick results in under 8 hr using Illumina sequencing systems

  • Achieves high, even coverage and accurate calls for PCR amplicons

  • Produces high-quality de novo assembly of small genomes

  • Allows scalability by processing and uniquely barcoding up to 384 samples in a single experiment

Specifications

Assay time ~5.5 hr from DNA extraction to normalized library. (Library prep time: ~90 minutes).
Automation capability Liquid Handling Robots
Description Prepare sequencing libraries for small genomes, PCR amplicons, and plasmids in 90 min, with a low DNA input requirement.
Hands-on time 15 min
Input quantity 1 ng genomic DNA (compatible with most large DNA genomes)
Mechanism of action Enzymatic fragmentation
Method De Novo Sequencing, Amplicon Sequencing
Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.
Nucleic acid type DNA
Specialized sample types Low-Input Samples, Single Cells
Species category Drosophila, Mammalian, Mouse, Yeast, Zebrafish, Human, Rat, Plant, Virus, Nematode, Bacteria
Systems MiSeq System, NextSeq 1000 System, MiniSeq System, NextSeq 550 System
Target insert size 300 bp–1.5 kb
Variant class Single Nucleotide Polymorphisms (SNPs)

Required Products

The Nextera XT DNA Library Preparation Kit offers a fast and easy workflow for preparing, quantifying, and normalizing libraries for sequencing small genomes, PCR amplicons, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA from a low amount of input DNA (1 ng), making this kit amenable for use with precious samples available in limited quantity.

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Applications

DRAGEN secondary analysis applications provide comprehensive coverage for many experiment types in a single platform, including whole-genome sequencing, exome sequencing, single-cell RNA-Seq, and methylation analysis.

View all DRAGEN secondary analysis apps

Software product portfolio

1
Lab

Clarity LIMS

Efficient sample tracking and workflow management for an optimized and efficient lab.

2
Run

Local run manager

Intuitive on-instrument run setup and monitoring

BaseSpace sequence hub

Streamlined cloud-based run setup and monitoring

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Analytics

DRAGEN

The most accurate and comprehensive secondary analysis for next generation sequencing optimized for efficiency

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Insights

Project recommendations

Instrument Recommended number of samples Read length
NextSeq 550 System

1 sample per run (high output; based on 30× coverage of a human genome)

Up to 2 × 150 bp
NovaSeq 6000 System

2–10 samples per run  (dual flow cell; based on 30× coverage of a human genome)

Up to 2 × 125 bp (rapid run) 
Up to 2 × 150 bp (high output)

Related applications and methods

Whole-genome sequencing

Whole-genome sequencing (WGS) delivers a comprehensive view, ideal for discovery applications. Newer sequencing systems perform WGS more rapidly than ever. 

Exome sequencing

Whole-exome sequencing (WES) is used to investigate protein-coding regions of the genome to uncover genetic influences on disease and population health. 

RNA sequencing

RNA-Seq uses NGS to analyze expression across the transcriptome, enabling scientists to detect known or novel features and quantify RNA. 

Documentation

Product literature
Support documentation

Compare

TruSight Cancer | Test Page Injection Illumina DNA Prep
Assay time ~5.5 hr from DNA extraction to normalized library. (Library prep time: ~90 minutes). ~3-4 hr (from DNA extraction to normalized library)
Automation capability Liquid Handling Robots Liquid Handling Robots
Description Prepare sequencing libraries for small genomes, PCR amplicons, and plasmids in 90 min, with a low DNA input requirement.

A fast, flexible library prep workflow that accommodates an assortment of sample types and DNA input amounts, allowing access to a wide range of applications, from human to microbial whole-genome sequencing and more. 
Hands-on time 15 min 1-1.5 hr
Input quantity 1 ng genomic DNA (compatible with most large DNA genomes) 1-500 ng DNA for small genomes (e.g. microbial), or 100-500 ng DNA for large genomes (e.g. human). (For blood and saliva, see the reference guide).
Mechanism of action Enzymatic fragmentation Bead-linked transposome
Method De Novo Sequencing, Amplicon Sequencing Shotgun Sequencing, Whole-Genome Sequencing, De Novo Sequencing, Amplicon Sequencing
Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together. Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations
Nucleic acid type DNA DNA
Specialized sample types Low-Input Samples, Single Cells Blood, Not FFPE-Compatible, Saliva
Species category Drosophila, Mammalian, Mouse, Yeast, Zebrafish, Human, Rat, Plant, Virus, Nematode, Bacteria Drosophila, Mammalian, Mouse, Yeast, Zebrafish, Human, Rat, Plant, Virus, Nematode, Bacteria, Any Species
Systems MiSeq System, NextSeq 1000 System, MiniSeq System, NextSeq 550 System MiSeq System, iSeq 100 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq X System, NovaSeq 6000 System, NovaSeq X Plus System, NextSeq 550 System
Target insert size 300 bp–1.5 kb ~350 bp
Variant class Single Nucleotide Polymorphisms (SNPs) Single Nucleotide Polymorphisms (SNPs), Gene Fusions, Loss of Heterozygosity (LOH), Somatic Variants, Chromosomal Abnormalities, Germline Variants, Structural Variants, Insertions-Deletions (indels), Copy Number Variants (CNVs)

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Figures

TruSight Cancer | Test Page Injection

TruSight Cancer | Test Page Injection

TruSight Cancer | Test Page Injection

Resources

TruSight Cancer | Test Page Injection

This video reviews the key steps in the Nextera library preparation process and provides some tips and tricks on how to achieve optimal results.

Thumbnail

Test Fragment

This video reviews the key steps in the Nextera library preparation process and provides some tips and tricks on how to achieve optimal results.

TruSight Cancer | Test Page Injection

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Infinium HTS iSelect-24 Kit (288 Samples, up to 700K)

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The Infinium HTS iSelect-24 288-Sample Kit contains 12 custom BeadChips designed to interrogate up to 700,000 custom targets and reagents to process 288 DNA samples through the Infinium HTS workflow.

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Infinium HTS iSelect-24 Kit (1152 Samples, up to 700K)

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The Infinium HTS iSelect-24 1152-Sample Kit contains 48 custom BeadChips designed to interrogate up to 700,000 custom targets and reagents to process 1152 DNA samples through the Infinium HTS workflow.

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Includes Avance Exchange support for the first year. Requires purchase of annual DRAGEN license.

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TruSight Oncology 500 HRD Kit (24 samples)

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TruSight Oncology 500 HRD Kit (includes HRD enrichment reagents. Does not include library prep or sequencing core reagents. HRD analysis license is required and sold separately.) Not available in Japan.

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Cloud: Microsoft Azure (1)

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This course is an introduction to Illumina sequencing technology and its possible applications for new customers. During 2 days of in-house training, customers will get an overview of each step of a RNA sequencing workflow; from considerations for experimental design, choice of sample preparation method and sequencer, to challenges of data analysis.

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Combined DNA and RNA workflow for comprehensive profiling of solid tumors with AmpliSeq™ for Illumina Focus Panel – Illumina Solution Center

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In-house EMEA training: This 3-day course is a full DNA and RNA sample- to -answer training for customers interested in biomarker analysis of 52 genes with known relevance to solid tumors. The course will cover integrated DNA/RNA workflow using AmpliSeq™ for Illumina Focus Panel and commercial reference standards, benchtop sequencing set-up and data analysis options for SNVs, indels, CNVs and gene fusions.

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FAQs

The Nextera XT DNA Library Preparation Kit offers a fast and easy workflow for preparing, quantifying, and normalizing libraries for sequencing small genomes, PCR amplicons, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA from a low amount of input DNA (1 ng), making this kit amenable for use with precious samples available in limited quantity.
This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

The Nextera XT DNA Library Preparation Kit offers a fast and easy workflow for preparing, quantifying, and normalizing libraries for sequencing small genomes, PCR amplicons, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA from a low amount of input DNA (1 ng), making this kit amenable for use with precious samples available in limited quantity.
This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

The Nextera XT DNA Library Preparation Kit offers a fast and easy workflow for preparing, quantifying, and normalizing libraries for sequencing small genomes, PCR amplicons, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA from a low amount of input DNA (1 ng), making this kit amenable for use with precious samples available in limited quantity.
This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

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NovaSeq 6000 Xp Workflow

NovaSeq 6000 Xp Workflow delivers the flexibility of independent lane loading and high library multiplexing per flow cell, maximizing sample throughput on a single run.

iSeq 100 Products

Library preparation kits and sequencing reagents compatible with the iSeq 100 benchtop sequencer.

NextSeq 1000/2000 Reagents

Reagents for the NextSeq 1000/2000 System feature easy-to-use cartridges and multiple flow cell configurations for flexible sequencing options.

iSeq 100 Reagents

Reagents for the iSeq 100 System feature a ready-to-use cartridge with one-channel SBS chemistry and CMOS technology for simple, low-cost sequencing.

NovaSeq 6000 Xp Workflow

NovaSeq 6000 Xp Workflow delivers the flexibility of independent lane loading and high library multiplexing per flow cell, maximizing sample throughput on a single run.

iSeq 100 Products

Library preparation kits and sequencing reagents compatible with the iSeq 100 benchtop sequencer.

NextSeq 1000/2000 Reagents

Reagents for the NextSeq 1000/2000 System feature easy-to-use cartridges and multiple flow cell configurations for flexible sequencing options.

iSeq 100 Reagents

Reagents for the iSeq 100 System feature a ready-to-use cartridge with one-channel SBS chemistry and CMOS technology for simple, low-cost sequencing.

Speak with a specialist

Reach out for information about our products and services, or get answers to questions about our technology.