The AmpliSeq for Illumina library preparation workflow requires a panel, library prep reagents (Library Plus), and indexes. Accessory products are also available for cDNA synthesis and sample identification.
AmpliSeq cDNA Synthesis for Illumina is required to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels.
AmpliSeq Library Equalizer for Illumina is an optional but recommended easy-to-use solution for normalizing libraries while using AmpliSeq for Illumina library prep methods.
AmpliSeq ERCC RNA Spike-In Mix for Illumina is a set of external RNA controls developed by the External RNA Controls Consortium (ERCC). The controls consist of a set of unlabeled, polyadenylated transcripts that can be used with AmpliSeq for Illumina assays with transcriptome panels to quantitate differential gene expression. Requires AmpliSeq ERCC RNA Companion Panel for Illumina (sold separately by Illumina).
AmpliSeq ERCC RNA Companion Panel for Illumina is intended for use with the AmpliSeq ERCC RNA Spike-In Mix for Illumina (sold separately by Illumina) to quantitate differential gene expression when performing AmpliSeq for Illumina assays with transcriptome panels.