Illumina DNA Prep with Exome 2.5 Enrichment offers a cost-effective, reliable solution for human whole-exome sequencing (WES). This kit provides:
A high-performance, fast, and reliable human whole-exome sequencing solution that includes a comprehensive, up-to-date exome enrichment panel.
Assay time
Hands-on time
Input quantity
Illumina DNA Prep with Exome 2.5 Enrichment offers a cost-effective, reliable solution for human whole-exome sequencing (WES). This kit provides:
Illumina DNA Prep with Exome 2.5 Enrichment is a whole-exome sequencing kit that includes library prep and hybridization reagents, clean up/size selection beads, and indexes. The kit also includes the Twist Bioscience for Illumina Exome 2.5 Panel that is smaller than the Illumina Exome Panel and has an improved target region coverage for variants reported in the public databases. In addition, mitochondrial genome coverage can be added easily with a spike-in panel.
This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.
Assay time | 6.5 hr |
---|---|
Automation details | Explore available automation methods |
Content specifications |
37.5 Mb coding content (≥ 99% of RefSeq, CCDS, ClinVar and ACMG pathogenic/likely pathogenic variants, COSMIC Cancer Gene Census), OMIM |
Hands-on time | ~2 hr |
Input quantity | 50-1000 ng high-quality genomic DNA (For direct blood and saliva, see reference guide) |
Mechanism of action | Bead-linked transposome and hybrid-capture chemistry |
Method | Target Enrichment, Exome Sequencing, Targeted DNA Sequencing |
Multiplexing | Pre-enrichment pooling of up to 12-plex is tested. Kit configuration supports multiplexing up to 192 samples. Up to 384 possible. |
Nucleic acid type | DNA |
On-target reads | ~ 90% based on average performance from internal testing |
Specialized sample types | Blood, Saliva |
Species category | Human |
Systems | NextSeq 2000 System, NextSeq 1000 System, NextSeq 550Dx in Research Mode, NovaSeq 6000 System, NextSeq 550 System |
Technology | Sequencing |
Uniformity | ≥ 91% (% coverage at 20× for 5 Gb) based on average performance from internal testing |
Variant class | Single Nucleotide Polymorphisms (SNPs), Loss of Heterozygosity (LOH), Somatic Variants, Germline Variants, Insertions-Deletions (indels), Copy Number Variants (CNVs) |
설명 | 비용 효율적이고 안정적인 인간 전장 엑솜 시퀀싱을 위한 최신 엑솜 콘텐츠의 집중 인리치먼트 프로브 패널을 사용하는 인리치먼트 워크플로우가 포함된 빠르고 사용하기 쉬운 라이브러리 준비입니다. |
Illumina DNA Prep with Exome 2.5 Enrichment kits include library prep, enrichment, enrichment probe panel, purification beads, and index adapter reagents. Choose a different index set if preferred.
For direct blood input, the Flex Lysis Reagent Kit is required.
The Infinium MIDI Heatblock Insert fits in the SciGene HybEx Microheating system and is required for the enrichment step.
The Illumina DNA Prep with Exome 2.5 Enrichment is a fast and comprehensive end-to-end whole-exome sequencing solution that enables support from sample to reporting.
Illumina DNA Prep with Exome 2.5 Enrichment
Instrument | Recommended number of samples | Read length |
---|---|---|
NextSeq 2000 System | Samples per run (by flow cell type): P2: 19, P3: 57 (based on 50× mean target coverage, 42M reads, 4.2 Gb of data) |
2 × 101 bp |
NovaSeq 6000 System | Samples per run by flow cell type: SP: 34, S1: 69, S2: 176, S4: 428 based on 50× mean target coverage, 42M reads, 4.2 Gb of data. Note: S4: 428 requires NovaSeq Xp workflow or additional indexes. |
2 × 101 bp |
Whole-exome sequencing is used to investigate protein-coding regions of the genome to uncover genetic influences on disease and population health.
Complex and genetic disease research
Comprehensive array and next-generation sequencing solutions to accelerate research of various genetic complex diseases.
Our sequencing and microarray technologies support a broad range of cancer genomics research applications, from DNA to RNA analysis, epigenetics, and more.
Literature is not currently available for this product.
Illumina DNA Prep with Exome 2.5 Enrichment | Illumina DNA Prep with Enrichment | |
---|---|---|
Assay time | 6.5 hr | ~6.5 hr |
Automation details | Explore available automation methods | Explore available automation methods |
Content specifications |
37.5 Mb coding content (≥ 99% of RefSeq, CCDS, ClinVar and ACMG pathogenic/likely pathogenic variants, COSMIC Cancer Gene Census), OMIM |
Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). Custom: 0.5 - 15 Mb genomic content of interest. Fixed panels: Content varies by panel. |
Hands-on time | ~2 hr | ~2 hr |
Input quantity | 50-1000 ng high-quality genomic DNA (For direct blood and saliva, see reference guide) | 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide). |
Mechanism of action | Bead-linked transposome and hybrid-capture chemistry | Bead-bound transposomes and hybrid-capture chemistry |
Method | Target Enrichment, Exome Sequencing, Targeted DNA Sequencing | Target Enrichment, Exome Sequencing, Custom Sequencing, Targeted DNA Sequencing |
Multiplexing | Pre-enrichment pooling of up to 12-plex is tested. Kit configuration supports multiplexing up to 192 samples. Up to 384 possible. | Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested. |
Nucleic acid type | DNA | DNA |
On-target reads | ~ 90% based on average performance from internal testing | ≥85% for exome panel (results from example data set; actual performance may vary) |
Specialized sample types | Blood, Saliva | Blood, Low-Input Samples, FFPE Tissue, Saliva |
Species category | Human | Other, Human |
Systems | NextSeq 2000 System, NextSeq 1000 System, NextSeq 550Dx in Research Mode, NovaSeq 6000 System, NextSeq 550 System | MiSeq System, iSeq 100 System, NextSeq 2000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System, NovaSeq X Plus System, NextSeq 550 System |
Technology | Sequencing | Sequencing |
Uniformity | ≥ 91% (% coverage at 20× for 5 Gb) based on average performance from internal testing | ≥90% (% coverage at 20x for 4 Gb; for exome panel) |
Variant class | Single Nucleotide Polymorphisms (SNPs), Loss of Heterozygosity (LOH), Somatic Variants, Germline Variants, Insertions-Deletions (indels), Copy Number Variants (CNVs) | Single Nucleotide Polymorphisms (SNPs), Loss of Heterozygosity (LOH), Somatic Variants, Germline Variants, Insertions-Deletions (indels), Copy Number Variants (CNVs) |
설명 | 비용 효율적이고 안정적인 인간 전장 엑솜 시퀀싱을 위한 최신 엑솜 콘텐츠의 집중 인리치먼트 프로브 패널을 사용하는 인리치먼트 워크플로우가 포함된 빠르고 사용하기 쉬운 라이브러리 준비입니다. |
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Libraries were constructed and enriched using Illumina DNA Prep with Exome 2.5 Enrichment with either the included Exome 2.5 Panel with a 1.5 hr or 16 hr hybridization time or the Illumina Exome Panel (IEP, 20020183) with a 1.5 hr hybridization time. Enriched libraries were sequenced on a NovaSeq 6000 System and analyzed using DRAGEN Enrichment App.
The bed files for the Exome 2.5 Panel and Exome Panel were compared to the listed public databases and the percent overlap was determined and reported.
Illumina DNA Prep with Exome 2.5 Enrichment using a 1.5-hr (orange) or 16-hr (blue) hybridization time shows high average percent coverage at ≥ 20× of targets from public databases.
Illumina DNA Prep with Exome 2.5 Enrichment, (S) Tagmentation Set B (96 Samples, 12-plex)
20077595
Includes Illumina DNA Prep with Enrichment library preparation and enrichment reagents, Twist Bioscience for Illumina Exome 2.0 Plus Panel, Illumina Purification Beads, and IDT for Illumina DNA/RNA UD Indexes Set B, Tagmentation for whole exome enrichment of 96 libraries (eight, 12-plex enrichment reactions).
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Illumina DNA Prep with Exome 2.5 Enrichment, (S) Tagmentation Set D (96 Samples, 12-plex)
20077596
Includes Illumina DNA Prep with Enrichment library preparation and enrichment reagents, Twist Bioscience for Illumina Exome 2.0 Plus Panel, Illumina Purification Beads, and IDT for Illumina DNA/RNA UD Indexes Set D, Tagmentation for whole exome enrichment of 96 libraries (eight, 12-plex enrichment reactions).
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Twist Bioscience for Illumina Mitochondrial Panel
20093180
Includes Twist Bioscience for Illumina Mitochondrial Panel, 32 ul of reagent for 96 samples (8 hybridization reactions with 12 samples per reaction). This panel can be used with the Exome 2.0 Plus Panel in the Illumina DNA Prep with Exome 2.0 Plus Enrichment kits, for combined enrichment of both human exome plus mitochondrial DNA from whole genome libraries. The Mitochondrial Panel is sold as an accessory for the Exome 2.0 Plus Panel products.
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Flex Lysis Reagent Kit (96 reactions)
20018706
Includes reagents for processing blood samples with the Illumina DNA Prep and Illumina DNA Prep with Enrichment. Purchase library prep, enrichment, enrichment probe panel, and index adapter reagents separately.
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Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)
20091654
Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)
20091656
Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)
20091658
Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)
20091660
Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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Infinium MIDI Heatblock Insert
BD-60-601
Heatblock insert for the SciGene Hybex Microheating system required for the Nextera Flex for Enrichment hybridization steps.
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Illumina Purification Bead, 100mL
20060057
Includes one, 100-ml container of beads for library clean-up and size selection. Purchase library prep and enrichment reagents, probe panels, and indexes separately.
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Illumina Purification Bead, 400mL
20060058
Includes four, 100-ml container of beads for library clean-up and size selection. Purchase library prep and enrichment reagents, probe panels, and indexes separately.
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Yes, gDNA input of 10-49 ng can be used. However, this protocol does not normalize final pre-enrichment library yields < 50 ng and, therefore, quantification and normalization of libraries before and after enrichment is required.
This protocol has been validated with fresh blood collected in EDTA collection tubes. Other anticoagulants may be used but are not guaranteed when using this kit.
This protocol is validated for saliva collected only in Oragene DNA Saliva collection tubes.
The Twist Bioscience for Illumina Mitochondrial Panel is an optional fixed content panel designed to cover the 16,659 base pairs (bp) and 37 genes of the mitochondrial genome (chrM) and allows enrichment and sequencing of mitochondrial DNA (mtDNA) variants. Use this panel as a spike-in panel in the Illumina DNA Prep with Exome 2.5 Enrichment protocol and add coverage of ChrM to your experiments.
The products previously known as Illumina DNA Prep with Exome 2.0 Plus Enrichment are now called Illumina DNA Prep with Exome 2.5 Enrichment (Cat. No. 20077595 and 20077596). The exome panel oligos have not changed, but the included indexes have been updated to Illumina DNA/RNA UD Indexes.
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