Illumina Total RNA Prep with Ribo-Zero Plus provides accurate measurement of gene and transcript abundance and detects both known and novel features in coding and multiple forms of noncoding RNA. Researchers can have confidence in discovery of alternative transcripts, gene fusions, and allele-specific expression. Additionally, the Illumina Stranded Total RNA Prep with Ribo-Zero Plus Microbiome delivers high-quality data for metatranscriptomics applications.