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3-4 days from sample to answer
Assay time
5 hr, 20 min
Hands-on time
Overview
The Nextera XT DNA Library Preparation Kit offers a fast and easy workflow for preparing, quantifying, and normalizing libraries for sequencing small genomes, PCR amplicons, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA from a low amount of input DNA (1 ng), making this kit amenable for use with precious samples available in limited quantity.
This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.
Specifications
| Assay time | 3-4 days from sample to answer |
|---|---|
| Hands-on time | 5 hr, 20 min |
| Method | Whole-Transcriptome Sequencing |
| Nucleic acid type | DNA |
| Systems | iScan System |
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Applications
The Nextera XT DNA Library Preparation Kit supports a low DNA input (1 ng) and offers high-quality sequencing-ready libraries for small genomes, PCR amplicons, and plasmids, in as little as 90 minutes.
Featured Workflow
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| Instrument | Recommended number of samples | Read length |
|---|---|---|
| Target Enrichment | 10 |
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Related applications and methods
NGS-based microbial sequencing methods include shotgun metagenomics, 16S rRNA sequencing, whole-genome, and de novo sequencing, and transcriptomics.
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NGS-based microbial sequencing methods include shotgun metagenomics, 16S rRNA sequencing, whole-genome, and de novo sequencing, and transcriptomics.
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High coverage, accurate calls
Nextera XT kit produces high and even coverage across a range of amplicon sizes with accurate variant calls as confirmed within the dbSNP database.
Resources
Library Prep (2)
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BaseSpace® HT Onsite Sequence Hub-4X+Rack is designed to support high- throughput instruments (e.g. HiSeq 4000) for on-site NGS data storage, analysis, and sharing.
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MiSeq FGx™ Upgrade Kit
SY-411-1002
MiSeq FGx Upgrade Kit contains all of the accessories needed to upgrade the MiSeq RUO to the MiSeq FGx, including MiSeq FGx Control Software and front panel containing MiSeq FGx product name.
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Cloud: Amazon Web Services (2)
NextSeq 500 to NextSeq® 550 Upgrade
SY-415-1003
Upgrade NextSeq 500 to NextSeq 550 and enable array scanning of CytoSNP- 850k, CytoSNP-12, HumanKaryomap-12, Infinium MethylationEPIC v1.0, and Infinium MethylationEPIC v2.0 BeadChips. Upgrade cost will cover upgrade onsite by FSE.
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NextSeq 500 to NextSeq® 550 Upgrade
SY-415-1003
Upgrade NextSeq 500 to NextSeq 550 and enable array scanning of CytoSNP- 850k, CytoSNP-12, HumanKaryomap-12, Infinium MethylationEPIC v1.0, and Infinium MethylationEPIC v2.0 BeadChips. Upgrade cost will cover upgrade onsite by FSE.
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Nextera XT DNA Library Preparation Kit
Offers a 90-min workflow to prepare DNA libraries for small genomes, PCR amplicons, plasmids, and cDNA sequencing, with a low DNA input requirement.
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