The Illumina Ribo-Zero Plus Microbiome rRNA Depletion Kit is a streamlined RNA-to-analysis solution for metatranscriptome sequencing of complex microbial samples, including stool.
Ribo-Zero Plus Microbiome rRNA Depletion Kit
Fast, efficient depletion of undesirable host and pan-bacterial rRNA from complex microbial samples (eg stool) for metatranscriptomics research.
Overview
Key features
Specifications
| Description | This product depletes abundant rRNA transcripts from complex microbial samples, allowing researchers to focus on high-value, informative portions of the metatranscriptome. This kit includes access to the BaseSpace Microbiome Metatranscriptomics app for simplified data analysis and visualization. |
|---|---|
| Input quantity | 25-1000 ng standard-quality total RNA |
| Mechanism of action | Enzymatic rRNA depletion, ligation-based addition of adapters and indexes |
| Method | Whole-Transcriptome Sequencing, Metatranscriptome Sequencing |
| Nucleic acid type | RNA |
| Specialized sample types | Microbiome Samples |
| Species category | Mouse, Human, Rat, Bacteria |
| Systems | NextSeq 2000 System, NextSeq 1000 System, NovaSeq 6000 System, NextSeq 550 System |
| Technology | Sequencing |
| Variant class | Novel Transcripts, Transcript Variants |
Applications
Example workflow
Prep
Ribo-Zero Plus Microbiome rRNA Depletion Kit
Project recommendations
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| NextSeq 550 System | Mid output: 2 High output: 8 (based on 25-50 million reads per sample) |
2 x 75 bp |
| NovaSeq 6000 System | Whole Transcriptome Analysis: SP: 16 S1: 32 S2: 82 S4: 200 samples per run (based on 25-50 million reads per sample) |
2 × 100 bp |
Related applications and methods
Human microbiome analysis with NGS
Analyze the human microbiome using NGS methods such as shotgun metagenomics, 16S rRNA metagenomics, and metatranscriptomics.
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Documentation
Product literature
Support documentation
A dedicated support section is not currently available for this product
Compare
| Ribo-Zero Plus Microbiome rRNA Depletion Kit | Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome | |
|---|---|---|
| Description | This product depletes abundant rRNA transcripts from complex microbial samples, allowing researchers to focus on high-value, informative portions of the metatranscriptome. This kit includes access to the BaseSpace Microbiome Metatranscriptomics app for simplified data analysis and visualization. |
For the preparation of whole-transcriptome sequencing-ready libraries from low RNA inputs and low-quality samples that supports a wide range of RNA-Seq applications. Includes Ribo-Zero Plus for single-tube depletion of rRNA and globin RNA from multiple species to facilitate rich transcriptome analyses. |
| Input quantity | 25-1000 ng standard-quality total RNA |
1–1000 ng total RNA for standard-quality RNA samples. 10 ng total RNA minimum recommended for optimal performance and FFPE samples |
| Mechanism of action | Enzymatic rRNA depletion, ligation-based addition of adapters and indexes | Enzymatic rRNA depletion, ligation-based addition of adapters and indexes |
| Method | Whole-Transcriptome Sequencing, Metatranscriptome Sequencing | Whole-Transcriptome Sequencing, Metatranscriptome Sequencing |
| Nucleic acid type | RNA | RNA |
| Specialized sample types | Microbiome Samples | Microbiome Samples, Low-Input Samples, FFPE Tissue |
| Species category | Mouse, Human, Rat, Bacteria | Mouse, Human, Rat, Bacteria |
| Systems | NextSeq 2000 System, NextSeq 1000 System, NovaSeq 6000 System, NextSeq 550 System | NextSeq 2000 System, NextSeq 1000 System, NovaSeq 6000 System, NextSeq 550 System |
| Technology | Sequencing | Sequencing |
| Variant class | Novel Transcripts, Transcript Variants | Single Nucleotide Polymorphisms (SNPs), Gene Fusions, Novel Transcripts, Transcript Variants |
Figures
Robust ribodepletion with the Ribo-Zero Plus Microbiome Depletion kit
The Ribo-Zero Plus Microbiome Depletion kit effectively depletes rRNA (5S, 16S, and 23S) levels for human and bacterial species in a single-tube reaction. Results are compared to Ribo-Zero Plus rRNA Depletion Kit and a commercially available ribodepletion kit from Company X. Two stool mixtures were tested. Sample 1 is a mixture containing both adult and infant human stool samples, while Sample 2 is a commercially available stool sample from Zymo (Cat no. 6331).
Resources
Unbiased Microbial Profiling with Metatranscriptomics
See how Drs. Tonya Ward, Emily Hollister, and Le François of Diversigen and DNA Genotek developed workflows for metatranscriptomic studies and read their views for this research area’s potentially impactful future.
Metatranscriptomics: Challenges and Opportunities
In this webinar, you'll hear from three leading experts in the field as they share their insights and key considerations on sample collection and processing, rRNA depletion, and bioinformatic analysis.
Illumina® Ribo-Zero Plus rRNA Microbiome depletion kit (96 Samples)
20072062
Includes Ribo-Zero Plus Microbiome depletion module for depletion of ribosomal RNA from diverse bacteria commonly found in microbiomes and targets included in the Ribo-Zero Plus kit for 96 samples. For complete kit including library prep reagents, order the Illumina Stranded Total RNA with Ribo-Zero Plus Microbiome kit.
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Illumina® Stranded Total RNA Prep with Ligation, Ribo-Zero Plus Microbiome (96 Samples)
20072063
Includes reagents and Ribo-Zero Plus Microbiome depletion module for preparing 96 libraries. Compatible only with IDT for Illumina RNA UD indexes, Ligation. Indexes sold separately.
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Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome
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Ribo-Zero Plus Microbiome rRNA Depletion Kit
Fast, efficient depletion of undesirable host and pan-bacterial rRNA from complex microbial samples (eg stool) for metatranscriptomics research.
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Specifications
Product Highlights
The Illumina Ribo-Zero Plus Microbiome rRNA Depletion Kit is a streamlined RNA-to-analysis solution for metatranscriptome sequencing of complex microbial samples, including stool.
- Perform deeper analysis and gain insights into microbial activity by reducing unwanted reads and increasing coverage of expressed bacterial RNA.
- Eliminate unwanted rRNA from bacteria common in the adult and infant human gut, as well as host RNA from human, mouse, using proven Ribo-Zero Plus enzymatic ribodepletion technology.
- Analyze complex metatranscriptomes with complimentary access to the BaseSpace Microbiome Metatranscriptomics app.
Read the data sheet to learn more about the metatranscriptomics applications.
Method:
Sequence Efficiently with:
Analyze Your Data with:
Robust ribodepletion with the Ribo-Zero Plus Microbiome Depletion kit. The Ribo-Zero Plus Microbiome Depletion kit effectively depletes rRNA (5S, 16S, and 23S) levels for human and bacterial species in a single-tube reaction. Results are compared to Ribo-Zero Plus rRNA Depletion Kit and a commercially available ribodepletion kit from Company X. Two stool mixtures were tested. Sample 1 is a mixture containing both adult and infant human stool samples, while Sample 2 is a commercially available stool sample from Zymo (Cat no. 6331).
Product Comparison
| Ribo-Zero Plus Microbiome rRNA Depletion Kit | Ribo-Zero Plus rRNA Depletion Kit | |
|---|---|---|
| Description | Depletion of abundant transcripts from host and bacterial rRNA for metatranscriptomics research. | Single-tube depletion of abundant transcripts from multiple species or transcripts |
| Input Quantity | Minimum 25 ng of total RNA, 50 ng recommended for optimal performance | 1-1000 ng total RNA for standard-quality RNA samples. 10ng total RNA minimum recommended for optimal performance and FFPE samples |
| System Compatibility Details | Library prep is designed to be compatible with all lllumina sequencing systems, and is recommended to be used with NextSeq 500, NextSeq 550, NextSeq 1000, NextSeq 2000, and NovaSeq 6000 Systems. | Library prep is designed to be compatible with all lllumina sequencing systems, and is extensively validated on the NextSeq 500, NextSeq 550 and NovaSeq 6000 Systems. |
| Strand Specificity | Stranded | Stranded |
| Method | Whole-Transcriptome Sequencing, Metatranscriptome Sequencing | Whole-Transcriptome Sequencing |
| Variant Class | Novel Transcripts, Transcript Variants | Gene Fusions, Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants |
| Species Category | Bacteria, Human, Mouse, Rat | Bacteria, Human, Mouse, Rat |
| Species Details | Depletion of abundant rRNA transcripts from complex bacterial (microbiome) samples including stool samples, in addition to all transcripts listed in Ribo-Zero Plus. | Depletion of abundant transcripts from multiple species including: human cytoplasmic & mitochondria rRNA, mouse rRNA, rat rRNA, E. coli and B. subtilis rRNA, human beta globin transcripts |
| System Compatibility | NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, NovaSeq 6000 | HiSeq 2500, HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, NovaSeq 6000 |
| Specialized Sample Types | Microbiome Samples | FFPE Tissue, Low-Input Samples |
| Nucleic Acid Type | RNA | RNA |
Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome
Streamlined RNA-Seq solution for clear and comprehensive analysis across the coding and non-coding transcriptome with exceptional study flexibility.
BaseSpace Sequence Hub
Data management and simplified bioinformatics for labs getting started and for rapidly scaling next-generation sequencing (NGS) operations.
Additional Resources
Unbiased Microbial Profiling with Metatranscriptomics
See how Drs. Tonya Ward, Emily Hollister, and Le François of Diversigen and DNA Genotek developed workflows for metatranscriptomic studies and read their views for this research area’s potentially impactful future.
See SpotlightMetatranscriptomics: Challenges and Opportunities
In this webinar, you'll hear from three leading experts in the field as they share their insights and key considerations on sample collection and processing, rRNA depletion, and bioinformatic analysis.
Watch Webinar