The NextSeq 1000 and NextSeq 2000 Systems deliver high-accuracy exome coverage for identification of true coding variants for applications like population genetics, genetic disease research, cancer studies, and more. An integrated DNA-to-results workflow requires minimal hands-on time and helps maximize efficiency in your lab.
NextSeq 1000 and NextSeq 2000 System applications and methods
Flexibility to do more
Fuel a broad range of applications, from targeted panels to whole-genome sequencing and more
Key applications and methods
Library prep
Illumina DNA Prep with Exome 2.5 Enrichment
A high-performance, fast, and complete whole-exome sequencing kit including library prep and hybridization reagents, a comprehensive exome probe panel, clean up/size selection beads, and indexes.
Sequence
Integrated reagent kits with reagent cartridge, flow cell, and resuspension buffer. Available in multiple flow cell configurations for adjustable output based on project needs.
Analyze
Aligns and calls variants from FASTQ, BAM, or CRAM files, outputting BAM and VCF files.
The NextSeq 1000 and NextSeq 2000 Systems power highly sensitive and accurate transcriptome analysis to quantify gene expression and identify known and novel transcript isoforms, gene fusions, and more.
Library prep
A simple, scalable, cost-effective, rapid single-day solution for analyzing the coding transcriptome using as little as 25 ng input of standard (nondegraded) RNA.
Sequence
Integrated reagent kits with reagent cartridge, flow cell, and resuspension buffer with Tween 20, offering multiple flow cell configurations for adjustable output based on project needs.
Analyze
Next-generation sequencing secondary analysis of RNA transcripts, including alignment, quantification, and fusion detection.
The NextSeq 1000 and NextSeq 2000 Systems enable researchers to assess the individual contributions of single cells in complex tissues by profiling the transcriptome.
Read single-cell RNA application note
Read 10x Genomics Chromium Single Cell Gene Expression technical note
Library prep
10x Genomics Chromium Single Cell Gene Expression
A solution that enables profiling of tens of thousands of cells with single-cell transcriptome 3’ gene expression and multiomic capabilities. Explore cellular heterogeneity, novel targets, and biomarkers with combined gene expression, surface protein expression, or CRISPR edits in each cell.
Reagents for measuring proteins at a single-cell level, with seamless integration into existing single-cell RNA sequencing workflows. TotalSeq Antibodies enable highly multiplexed single-cell protein studies for novel applications in precision medicine, oncology, immunology, neuroscience, and stem cell research.
Sequence
Integrated reagent kits with reagent cartridge, flow cell, and resuspension buffer with Tween 20, offering multiple flow cell configurations for adjustable output based on project needs.
Analyze
DRAGEN Single-Cell RNA Pipeline
Integrated analysis pipeline enables users to turn base calls into a cell-by-gene expression matrix with a single touchpoint. Compatible with a variety of third-party sample preparation kits. Available on the NextSeq 2000 System and the DRAGEN server.
SeqGeq Software by FlowJo, LLC
Import and analyze gene expression data or list of genes.
Multiomics multiplies your discovery power
The P3 flow cell on the NextSeq 2000 System is an excellent fit for multiomics studies. Use multiomics to better connect genotype to phenotype and obtain a full cellular readout not found through single-application approaches. Learn about multiomics at a glance.
Multiomics Multiplies Your Discovery Power
Your email address is never shared with third parties.
More applications and methods
16S metagenomic sequencing
Sequence the 16S ribosomal RNA (rRNA) gene with a culture-free method to identify and compare bacteria from complex, difficult-to-study microbiomes or environments.
Shotgun metagenomic sequencing
Sequence thousands of organisms in parallel to identify unculturable or low-abundance microbes, or evaluate microbial diversity.
Amplicon sequencing
Perform ultradeep sequencing of PCR amplicons with cost-effective analysis of up to hundreds of target genomic regions in one assay.
Small whole-genome sequencing
Sequence the entire genome of a bacterium, virus, or other microbe for applications in public health, infectious disease surveillance, molecular epidemiology studies, and environmental metagenomics.
ATAC-Seq
Determine chromatin accessibility across the genome by sequencing regions of open chromatin.
Spatial transcriptomics
Detect transcriptional activity to resolve mRNA expression at the cellular level in structurally preserved tissues.
ChIP-Seq
Analyze protein interactions with DNA for genome-wide surveys of gene regulation.
Target enrichment
Use sequence-specific hybridization to analyze genomic regions of interest.
ctDNA sequencing
Use next-generation sequencing for sensitive and specific detection of low levels of circulating tumor DNA (ctDNA) in the bloodstream.
Targeted DNA sequencing
Focus time, expenses, and analysis on sequencing a subset of genes or genome regions of research interest.
Immune repertoire analysis
Identify unique receptor variants to map the vast repertoire of immune cells capable of recognizing a seemingly boundless array of targets.
Targeted RNA sequencing
Select and sequence specific transcripts of interest for gene expression profiling studies.
Methylation sequencing
Enhance epigenetic studies with high-coverage density and flexibility enabled by sequencing-based DNA methylation analysis.
Total RNA sequencing
Analyze coding plus multiple forms of noncoding RNA for a comprehensive picture of the transcriptome.